Laser Spectroscopy - Chemometrics

Etablissement : Brest University (UBO)
Affectation de recherche : OPTIMAG : Optic and Magnetic Laboratory,

Equipe(s) : Optical Team
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These studies focus on the analyse of trace organic pollutants in water by laser-induced molecular fluorescence. The excitation is done by a Nd:YAG tuneable laser.

A new methods using Photo Induced Fluorescence has been recently published.

Environmental applications have been conducted in freshwater and seawater :

  • Endocrine disruptors (phthalates and phenolics)
  • Bisphenol A by Direct Laser Photo-Induced Fluorescence (DL-PIF)
  • Polycyclic Aromatic Hydrocarbons (PAHs) a family of organic pollutants carcinogens whose total concentration is limited to 0.2 mg/L in tap water. For the 6 regulated HPA We have obtained by direct analysis good detection limits, especially for benzo [a] pyrene with a DL of 0.7 ng/L. In the case of river water, the fluorescence of organic matter (humic acids) masks the compound. Using a time shift allows to reduce the matrix signal.

  • Pesticides
    - Anilides and phenylureas, by Direct Laser Photo-Induced Fluorescence (DL-PIF).
    - Carbamates by laser fluorescence  in different matrix. For example, for carbaryl the concentration limit for drinking water is 100 ng/L (500 ng / L for the sum of all pesticides). The use of a time delay of 6 ns can reduced the fluorescence of the matrix and achieve a detection limit of 10 ng/L.


1 – Analyze of oxadiazon herbicide in natural waters by a micellar-enhanced photo-induced fluorescence.
J. P. Bakhoum, O. M. A. Mbaye, P. A. Diaw, L. Cisse, M. Mbaye, M. D. Gaye-Seye, A. Coly, B. Le Jeune, P. Giamarchi.
Analytical Sciences, 2020, Vol 20, N° 4, 447-452.

2 – Development of a new automatic on-site detector of pesticides in natural waters by photo induced fluorescence, application to three phenylurea and benzoylurea herbicide.
J. P. Bakhoum, O. M. A. Mbaye, P. A. Diaw, M. Mbaye, L. Cisse, M. D. Gaye-Seye, J-J. Aaron, A. Coly, B. Le Jeune, P. Giamarchi.
Spectrochemica Acta Part A, 2019, Vol 214, 285-293.

3 - Ultraviolet photo-induced fluorescence followed by laser excitation (UV-PIF-LE) for the determination of herbicides in natural waters.
J. P. Bakhoum, O. Mbaye, P. Diaw, M. Mbaye, L. Cisse, D. Gaye-Seye, J.J. Aaron, A. Coly, B. Le Jeune, P. Giamarchi.
Analytical Letters, 2019, Vol 52, 2782-2793.

4 – Combination of photo-induced fluorescence and GC-MS for elucidating the photo degradation mechanisms of diflubenzuron and fenuron pesticides.
P. A. Diaw, O. M. A. Mbaye, D. D. Thiaré, N. Oturan, M. D. Gaye-Seye, A. Coly, B. Le Jeune, P. Giamarchi, M. A. Oturan, J.-J. Aaron.
Luminescence, 2019, Vol 34, 465-471.

5 - Development of online automatic detector of hydrocarbons and suspended organic matter by simultaneously acquisition of fluorescence and scattering
Mbaye M., Diaw P.A., Gaye-Saye, D. Le Jeune B., Cavalin G., Denis L., Aaron J.J. Delmas R., Giamarchi P.
Spectrochemica Acta Part A, 2018, Vol 192, 117-127.

6 - A new direct laser photo-induced fluorescence method coupled on-line with liquid chromatographic separation for the simultaneous determination of anilides pesticides.
Mbaye O., Maroto A., Gaye-Seye M.D., Stephan L., Deschamps L., Aaron J.J., Giamarchi P.
Talanta, 2015, Vol 132, 909-914.

7 – Determination of phenylurea pesticides by direct laser photo-induced fluorescence.
Diaw P.A., Maroto A., Mbaye O.M.A., Gaye-Seye M.D., Stephan L., Coly A., Deschamps L. , Tine A. , Aaron J.J. , Giamarchi P. 
Talanta, 2013, Vol 116, 569-574. .
Published paper:

8 – Direct Laser Photo-Induced Fluorescence Determination of Bisphenol A.
Maroto A., Kissingou P., Diascorn A., Benmansour B., Deschamps L., Stephan L., Cabon J.Y., Giamarchi P.
Analytical and Bioanalytical Chemistry, 2011, Vol 401, 3011-3017.
Published paper:

9 – Spectroscopic properties and laser induced fluorescence determination of some endocrine disrupting compounds
Benmansour .B, Stephan L., Cabon J.Y., Deschamps L., Giamarchi P.,
Journal of fluorescence, 2011, Vol 21, N°3, 843-850.

10 – Simulations of accidental coal immersion
Jaffrennou C., Stephan L., Giamarchi P., Cabon J.Y., Burel-Deschamps L., Bautin F. , Thomas A., Le Floch S.,
Marine Pollution Bulletin, 2007, Vol 54, N° 17, 1932 – 1939.

11 – Direct fluorescence monitoring of coal organic matters released in seawater.
Jaffrennou C., Stephan L., Giamarchi P., Cabon J.Y., Burel-Deschamps L., Bautin F.
Journal of fluorescence, 2007, Vol 17, N°5, 564-572.

12 – Laser induced fluorescence detection of carbamates traces in water.
Burel L., Giamarchi P., Stephan L., Lijour Y., Le Bihan A.
Journal of Fluorescence, 2006, Vol 16, N°2, 177-183.

13 – Molecular and atomic ultra traces analysis by laser induced fluorescence with OPO system and ICCD camera...
Burel L., Giamarchi P., Stephan L., Lijour Y., Le Bihan A.,
Talanta, 2003, Vol 60, 295-302.

14 - Laser-induced fluorescence with OPO system. Part I: Optimisation of the analytical system by use of experimental design methodology. Application to the direct quantification of traces of benzo[a]pyrene.
Giamarchi P., Burel L., Stephan L., Lijour Y., Le Bihan A.
Analytical and Bioanalytical Chemistry, 2002, Vol 374, N° 3, 490-497.

15 – Multicomponent determination of poly aromatic hydrocarbons mixture by direct fluorescence measurements.
Giamarchi P., Stephan L., Salomon S., Le Bihan A.
Journal of Fluorescence, 2000, Vol 10, N°4, 393-402.