Laser Spectroscopy - Chemometrics

Etablissement : Brest University (UBO)
Affectation de recherche : OPTIMAG : Optic and Magnetic Laboratory,

Equipe(s) : Optical Team
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Tél.+33 298016476

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We study by fluorescence spectroscopy the physicochemical properties of liposomes membranes composed of cationic phospholipids. These cationic lipids are synthesized by the "synthetic vectors" team (Jaffrès Pr) and consist in two fatty chains, a spacer and a cationic head with the ability to complex the DNA.

By fluorescence anisotropy we study the membrane viscosity and the phase transition temperature depending on the membrane composition and on the addition of colipids.

By FRET we have evaluate the fusion rate of liposomes
We have also conducted experiments on transfection efficiency (INSERM U613, Pr Lehn and Pr Férec).











1 - Biophysical properties of cationic lipophosphoramidates: vesicle morphology, bilayer hydration and dynamics
Loizeau D., Jurkiewicz P., Aydogan G., Philimonenko A., Mahfoudhi S., Hozák P., Maroto A., Couthon-Gourvès H., Jaffrès P.A., Deschamps L., Giamarchi P., Hof M.
Colloids and Surfaces B: Biointerfaces, 2015.
Published article :

2 – Functionalized Phospholipid Molecular Platform: Use for Production of Cationic Fluorescent Lipids.
Berchel M., Haelters J.P., Afonso D., Alicia M., Deschamps L., Giamarchi P., Jaffrès P.A.
European Journal of Organic Chemistry, 2014, 1076-1083. 
Published article :

3 – Physicochemical properties of cationic lipophosphoramidates with an arsonium head group and various lipid chains: a structure-activity approach.
Loizeau D., Le Gall T., Mahfoudhi S., Berchel M., Maroto A., Yaouanc J.J., Jaffrès P.A., Lehn P., Deschamps L., T, Giamarchi P.
Biophysical Chemistry, 2013, Vol 171, 46-53. 
Published article :

4 – Cationic Lipo-thiophosphoramidates for in vitro gene delivery: synthesis, physico-chemical characterizations and transfection assays - comparison with lipo-phosphoramidates.
Fraix A., Montier T., Carmoy N., Loizeau D, Burel-Deschamps L, Le Gall T, Giamarchi P, Couthon-Gourvès H, Haelters J.P., Lehn P, Jaffrès P.A.
Organic & Biomolecular Chemistry, 2011, Vol 9, 2422-2432. 
Published article :

5 – A novel cationic lipophosphoramide with di-unsaturated lipid chains: synthesis, physico-chemical properties and transfection activities.
Le Gall T., Loizeau D., Picquet E., Carmoy N., Yaouanc J.J., Deschamps L., Delépine P., Giamarchi P., Jaffrès P. A., Lehn P., Montier T.,
Journal of Medicinal Chemistry, 2010, Vol 53, N°4, 1496-1508.
Published article :

6 – Fluorescence study of lipid-based DNA carriers properties: Influence of cationic lipid chemical structure.
Burel-Deschamps L., Mével M., Loizeau D., Clément J.C., Jaffrès P.A., Giamarchi P.,
Journal of fluorescence, 2008, Vol 18, N°5, 835-841. 
Published article :

7 – Lipophosphoramidate as lipidic part of lipospermines for gene delivery.
Lamarche F., Mével M., Montier T., Burel-Deschamps L., Giamarchi P., Tripier R., Le Gall T., Cartier d., Lehn P., Clément J.C., Jaffrès P.A.,
Bioconjugate Chemistry, 2007, Vol 18, N°5, 1575-1582. 
Published article :