Charlie Kersuzan presents his work on depth imaging of organoids without labeling using full-field optical coherence tomography. ContenThe Biof team at LP2N has developed an encapsulation technique that embeds cells within alginate microspheres (≈300 µm diameter), enabling 3D growth and the formation of multicellular agregates, also known as organoids, from various cell types. FF-OCT is well suited for imaging these live samples due to its optical sectioning capability, low phototoxicity, and ability to image several hundred microns deep. I developed a new image acquisition strategy that simplifies the setup and reduces imaging time without degrading image quality. I will present results obtained on different sample types, including dynamic FF-OCT imaging of organoids, a recently developed acquisition method that captures the cellular activity without any fluorescent markers, by analyzing the cells fluctuations over time.